Transforming Brain Imaging: The Revolutionary NeuraLeap Module
Advancing Imaging with NeuraLeap
The NeuraLeap digital focusing module, an innovation for Ultima 2Pplus Multiphoton Microscopes, has revolutionized the field of imaging. Offering rapid imaging across various depths, NeuraLeap enhances the speed and flexibility of imaging, thus providing a superior temporal resolution in brain imaging. It allows for instantaneous switching between optical planes due to its extended depth-of-field laser excitation module, equipped with an ultrafast Digital Micromirror Device (DMD) chip.
NeuraLeap can define multiple excitation planes of diverse sizes, enabling researchers to selectively image larger volumes in a single optical plane. The rapid switching of planes without any wait time for the Z-device boosts the rate of scanning samples in Z. This also eliminates any additional delay for larger jumps. Furthermore, planes can be randomly selected for imaging, providing researchers with more freedom and flexibility in data collection.
Exploring Larger Volumes with NeuraLeap
The module can establish an elongated illumination spot that excites fluorophores in planes up to 150 microns thick with a 16x objective. This feature allows resonant-rate imaging of volumes in areas where optical signal mixing isn’t a concern. The larger illumination plane increases frame rates for volumes and prevents signal changes due to Z motions in samples, making data collection more reliable.
NeuraLeap can generate two discrete imaging spots along its full depth-of-field, allowing users to select different planes of interest to image at resonant-rate speeds. This dual-plane imaging feature enables the imaging of cells across large distances with high temporal precision, which is highly beneficial for more complex applications.
Integration with Bruker’s Flagship System
The NeuraLeap module is specifically engineered to be used with Bruker’s flagship all-optical multiphoton system for imaging and optical manipulations. This module’s unique features make it a valuable tool for researchers and professionals working with detailed and rapid imaging.
Enabling Real-Time Transmission Imaging
NeuraLeap provides the groundbreaking imaging speed required to access different layers of the cortex, a leap forward compared to traditional focus methods. The new module provides a three orders of magnitude improvement of switching speed between discrete imaging planes. This advancement will be especially useful for correlating the activity of neuronal circuits across cortical layers, enabling researchers to more comprehensively and precisely investigate larger neural network functions.
According to researchers, the Ultima 2Pplus microscopes, equipped with the NeuraLeap module, are continually enabling innovations to fulfill neuroscientists’ ultimate desire to image neural activity at the speed of real-time transmission. This marks another significant advancement in neuroscience research, along with recent neurotransmitter and calcium indicator improvements. With these advancements, it will be particularly exciting to see what researchers will discover using this new technology.
The NeuraLeap module is a ground-breaking addition to the field of neuroscience and imaging. It allows for rapid and detailed imaging across various depths, providing researchers with the tools they need to investigate larger neural network functions. The module’s ability to define multiple excitation planes of diverse sizes and switch between them instantaneously opens up new possibilities for research and data collection. With the integration of the NeuraLeap module, the Ultima 2Pplus Multiphoton Microscopes continue to enable innovations and push the boundaries of neuroscience research.
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